FRET Study of the Binding of HIV-1 RRE RNA to Rev Peptide

Midshipman Researcher(s): 1/C Jade M. DeSpain

Adviser(s): Professor Katherine Truex

Poster #85

In the viral life cycle of HIV-1, binding of the Rev protein to the Re v Response Element (RRE) RNA to form a complex is essential for viral RNA to be exported from the cell nucleus. The m6A posttranscriptional modification is known to regulate gene expression and modulate infectivity of viral RNAs. This study measures binding of an RNA hairpin that contains the high-affinity Rev-binding site to a peptide that contains the 17 amino acid binding region of the Rev protein as a model of the RRE-Rev binding site. Binding is measured via single molecule fluorescence resonance energy transfer (FRET) spectroscopy.

Full Size Physics #85